Embryology
Ahmad reza raji; shadi Hashemnia; Ahmad ail Mohammadpour; Abolghasem Nabipour; zohre saddatfar
Articles in Press, Accepted Manuscript, Available Online from 05 May 2024
Abstract
Ostrich (Struthio camelus) is an important wild species highlighted in national and international livestock. This research was conducted to analyze the development of the ostrich respiratory system in fetal and embryonic stages. A total of 50 fertile ostrich eggs were collected from commercial farms. ...
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Ostrich (Struthio camelus) is an important wild species highlighted in national and international livestock. This research was conducted to analyze the development of the ostrich respiratory system in fetal and embryonic stages. A total of 50 fertile ostrich eggs were collected from commercial farms. They were incubated at 36–37°C and 25 ± 2% humidity for 40 days. Sections were taken in 13, 22, 26, 30, 36, and 42 days of incubation from the lung and the cranial, middle, and caudal parts of the neck after decapitation of ostrich embryos and blood drainage. After fixation, processing, blocking, and sectioning, all samples were stained by hematoxylin and eosin, Alcian blue (AB), Van Gieson, and Periodic acid-Schiff (PAS) techniques.It was concluded that the trachea in the 13-day-old embryo (E13) and goblet cells (PAS-positive and AB-positive) had incomplete rings of hyaline cartilages and differentiation of mesenchymal to the loose connective tissue.Bronchial stage of the lung in the E22, pseudo-glandular stage in the E26, and parabrachial and air capillary stage in the E30 were also observed. The information obtained here will be useful for diagnosing the pathologies affecting this vital system and results in improving industrial breeding management.
Food Hygiene
Arezo Moghtaderi; Ahmadreza Raji; Saeid Khanzadi; Abolghasem Nabipour
Volume 10, Issue 4 , December 2019, , Pages 357-360
Abstract
Nowadays, the consumption of meat and meat products has been increased with modern manufacturing techniques. Due to the economic value, the likelihood of using unauthorized tissue is possible in meat products. The aim of this study was to apply morphological methods for detection of unauthorized tissues ...
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Nowadays, the consumption of meat and meat products has been increased with modern manufacturing techniques. Due to the economic value, the likelihood of using unauthorized tissue is possible in meat products. The aim of this study was to apply morphological methods for detection of unauthorized tissues in meat sausage. In this study, a total number of 20 samples of different types of sausages were randomly collected from markets, in north-east of Iran. Each sample was divided into three equal parts and three paraffin-embedded blocks were prepared from each part (180 blocks). Then the sections were stained using Hematoxylin and Eosin, Masson’s trichrome, Periodic acid- Schiff/Alcian blue and Verhoeffe/Van Gieson. A total number of 720 slides were observed using a light microscope. This research showed the use of unauthorized tissues in the sausages which was detected by histological methods. We observed authorized tissues like skeletal muscle fiber (100%), fat tissue (100%) and plant material (97.70%). A wide range of unauthorized tissues were detected including dense connective tissue (6.66%), cartilage (28.30%), bone (8.30%), skin (51.60%), smooth muscle (1.66%) and blood vessels (11.66%). The results of this study confirmed the use of unauthorized tissue in meat sausages in Iran and concluded that the histological methods especially Masson’s trichrome staining are a practical technique for routine assessment of authenticity and quality of sausage to protect the consumers from adulteration.
